pP{TargetB} Features |
|
1-235 |
3'P end |
296-329 |
FRT |
336-413 |
polylinker |
414-437 |
I-CreI site |
453-3674 |
mini-white |
3690-3723 |
FRT |
3760-4334 |
5'P end |
4335-6377 |
pUC backbone (AmpR) |
pP{TargetB} Sequence |
CATGATGAAATAACATAAGGTGGTCCCGTCGGCAAGAGACATCCACTTAACGTATGCTTG CAATAAGTGCGAGTGAAAGGAATAGTATTCTGAGTGTCGTATTGAGTCTGAGTGAGACAG CGATATGATTGTTGATTAACCCTTAGCATGTCCGTGGGGTTTGAATTAACTCATAATATT AATTAGACGAAATTATTTTTAAAGTTTTATTTTTAATAATTTGCGAGTACGCAAAGctag agttgttggttggcacaccacaaatatactgttgccgagcacaattccgctcgacGAAGT TCCTATTCTCTAGAAAGTATAGGAACTTCggatccAGATCTGCTAGCGGCCGCATGCCGC GGACGCGTAGGCCTGCAGGAATTCGATTCGACCTCGAGGGGGGGCCCGGTACCCAAAACG TCGTGAGACAGTTTGGTcaattctagtatgtatgtaagttaataaaacccatttttgcgg aaagtagataaaaaaaacattttttttttttactgcactggatatcattgaacttatctg atcagttttaaatttacttcgatccaagggtatttgatgtaccaggttctttcgattacc tctcactcaaaatgacattccactcaaagtcagcgctgtttgcctccttctctgtccaca gaaatatcgccgtctctttcgccgctgcgtccgctatctctttcgccaccgtttgtagcg ttacctagcgtcaatgtccgccttcagttgcactttgtcagcggtttcgtgacgaagctc caagcggtttacgccatcaattaaacacaaagtgctgtgccaaaactcctctcgcttctt atttttgtttgttttttgagtgattggggtggtgattggttttgggtgggtaagcagggg aaagtgtgaaaaatcccggcaATGGGCCAAGAGGATCAGGAGCTATTAATTCGCGGAGGC AGCAAACACCCATCTGCCGAGCATCTGAACAATgtgagtagtacatgtgcatacatctta agttcacttgatctataggaactgcgattgcaacatcaaattgtctgcggcgtgagaact gcgacccacaaaaatcccaaaccgcaatcgcacaaacaaatagtgacacgaaacagatta ttctggtagctgtgctcgctatataagacaatttttaagatcatatcatgatcaagacat ctaaaggcattcattttcgactacattcttttttacaaaaaatataacaaccagatattt taagctatgcacaaaaaataaataaaagtataaacctacttcgtaggatacttcgttttg ttcggggttagatgagcataacgcttgtagTTGATATTTGAGATCCCCTATCATTGCAGG GTGACAGCGGACGCTTCGCAGAGCTGCATTAACCAGGGCTTCGGGCAGGCCAAAAACTAC GGCACGCTCCTGCCACCCAGTCCGCCGGAGGACTCCGGTTCAGGGAGCGGCCAACTAGCC GAGAACCTCACCTATGCCTGGCACAATATGGACATCTTTGGGGCGGTCAATCAGCCGGGC TCCGGATGGCGGCAGCTGGTCAACCGGACACGCGGACTATTCTGCAACGAGCGACACATA CCGGCGCCCAGGAAACATTTGCTCAAGAACGgtgagtttctattcgcagtcggctgatct gtgtgaaatcttaataaagggtccaattaccaatttgaaactcagTTTGCGGCGTGGCCT ATCCGGGCGAACTTTTGGCCGTGATGGGCAGTTCCGGTGCCGGAAAGACGACCCTGCTGA ATGCCCTTGCCTTTCGATCGCCGCAGGGCATCCAAGTATCGCCATCCGGGATGCGACTGC TCAATGGCCAACCTGTGGACGCCAAGGAGATGCAGGCCAGGTGCGCCTATGTCCAGCAGG ATGACCTCTTTATCGGCTCCCTAACGGCCAGGGAACACCTGATTTTCCAAGCCATGGTGC GGATGCCACGACATCTGACCTATCGGCAGCGAGTGGCCCGCGTGGATCAGGTGATCCAGG AGCTTTCGCTCAGCAAATGTCAGCACACGATCATCGGTGTGCCCGGCAGGGTGAAAGGTC TGTCCGGCGGAGAAAGGAAGCGTCTGGCATTCGCCTCCGAGGCACTAACCGATCCGCCGC TTCTGATCTGCGATGAGCCCACCTCCGGACTGGACTCATTTACCGCCCACAGCGTCGTCC AGGTGCTGAAGAAGCTGTCGCAGAAGGGCAAGACCGTCATCCTGACCATTCATCAGCCGT CTTCCGAGCTGTTTGAGCTCTTTGACAAGATCCTTCTGATGGCCGAGGGCAGGGTAGCTT TCTTGGGCACTCCCAGCGAAGCCGTCGACTTCTTTTCCTACGTGGGTGCCCAGTGTCCTA CCAACTACAATCCGGCGGACTTTTACGTACAGGTGTTGGCCGTTGTGCCCGGACGGGAGA TCGAGTCCCGTGATCGGATCGCCAAGATATGGCGACAATTTTGCCATTAGCAAAGTAGCC CGGGATATGGAGCAGTTGTTGGCCACCAAAAATCTGGAGAAGCCACTGGAGCAGCCGGAG AATGGGTACACCTACAAGGCCACCTGGTTCATGCAGTTCCGGGCGGTCCTGTGGCGATCC TGGCTGTCGGTGCTCAAGGAACCACTCCTCGTAAAAGTGCGACTTATTCAGACAACGAAG ATAAATAAATTTCATTTAACTCGCGAATATTAATGAGATGCGAGTAACATTTTAATTTGC AGATGGTTGCCATCTTGATTGGCCTCATCTTTTTGGGCCAACAACTCACGCAAGTGGGTG TGATGAATATCAACGGAGCCATCTTCCTCTTCCTGACCAACATGACCTTTCAAAACGTCT TTGCCACGATAAATGTGTTCACCTCAGAGCTGCCAGTTTTTATGAGGGAGGCCCGAAGTC GACTTTATCGCTGTGACACATACTTTCTGGGCAAAACGATTGCCGAATTGCCGCTTTTTC TCACAGTGCCACTGGTCTTCACGGCGATTGCCTATCCGATGATCGGACTGCGGGCCGGAG TGCTGCACTTCTTCAACTGCCTGGCGCTGGTCACTCTGGTGGCCAATGTGTCAACGTCCT TCGGATATCTAATATCCTGCGCCAGCTCCTCGACCTCGATGGCGCTGTCTGTGGGTCCGC CGGTTATCATACCATTCCTGCTCTTTGGCGGCTTCTTCTTGAACTCGGGCTCGGTGCCAG TATACCTCAAATGGTTGTCGTACCTCTCATGGTTCCGTTACGCCAACGAGGGTCTGCTGA TTAACCAATGGGCGGACGTGGAGCCGGGCGAAATTAGCTGCACATCGTCGAACACCACGT GCCCCAGTTCGGGCAAGGTCATCCTGGAGACGCTTAACTTCTCCGCCGCCGATCTGCCGC TGGACTACGTGGGTCTGGCCATTCTCATCGTGAGCTTCCGGGTGCTCGCATATCTGGCTC TAAGACTTCGGGCCCGACGCAAGGAGTAGccgacatatatccgaaataactgcttgtttt ttttttttaccattattaccatcgtgtttactgtttattgccccctcaaaaagctaatgt aattatatttgtgccaataaaaacaagatatgacctatagaatacaaaaaaaaaaaaaaa aaaaaaaaaaaaaactcgatcgagtcgacGAAGTTCCTATTCTCTAGAAAGTATAGGAAC TTCggatccaaacctagtaattaattcggctgctgctctAAACGACGCATTTCGTACTCC AAAGTACGAATTTTTTCCCTCAAGCTCTTATTTTCATTAAACAATGAACAGGACCTAACG CACAGTCACGTTATTGTTTACATAAATGATTTTTTTTACTATTCAAACTTACTCTGTTTG TGTACTCCCACTGGTATAGCCTTCTTTTATCTTTTCTGGTTCAGGCTCTATCACTTTACT AGGTACGGCATCTGCGTTGAGTCGCCTCCTTTTAAATGTCTGACCTTTTGCAGGTGCAGC CTTCCACTGCGAATCATTAAAGTGGGTATCACAAATTTGGGAGTTTTCACCAAGGCTGCA CCCAAGGCTCTGCTCCCACAATTTTCTCTTAATAGCACACTTCGGCACGTGAATTAATTT TACTCCAGTCACAGCTTTGCAGCAAAATTTGCAATATTTCATTTTTTTTTATTCCACGTA AGGGTTAATGTTTTCAAAAAAAAATTCGTCCGCACACAACCTTTCCTCTCAACAAGCAAA CGTGCACTGAATTTAAGTGTATACTTCGGTAAGCTTCGGCTTTCGACGGGACCACCTTAT GTTATTTCATCATGggccagacccacgtagtccagcggcagatcggcggcggagaagtta agcgtctccaggatgaccttgcccgaactggggcacgtggtgttcgacgatgtgcagcta atttcgcccggctccacgtccgcccattggttaatcagcagaccctcgttggcgtaacgg aaccatgagaggtacgacaaccatttgaggtatactggcaccgagcccgagttcaagaag aaggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagt cagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctcc ctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctccct tcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtc gttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcctta tccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagca gccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaag tggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaag ccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggt agcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaa gatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaaggg attttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatga agttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgctta atcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactc cccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatg ataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccgga agggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgt tgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccatt gctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcc caacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttc ggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggca gcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgag tactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcg tcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaa cgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaa cccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtga gcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttga atactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatg agcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacattt ccccgaaaagtgccacctgcgaagtcttagagccagatatgcgagcacccggaagctcac gatgagaatggccagac |
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